Make sure your Bioformats plugin is installed and up to date (5.7.2 recomended).Ĭlick here to view the Volocity User Guide IMARISĬlick here to download the IMARIS Reference Guide. To quantify the colocalization of the CRMP2 and pericentrin proteins, Pearson Correlation Coefficient (PCC) was calculated using the ZEN Black software (Carl Zeiss).
tif converter for Axioscan files is implemented as a Fiji/ImageJ macro. Intensity profiles of selected molecular signals in the confocal images were generated using the ZEN lite 2.1 and were replotted using the GraphPad Prism software. Click here for download, installation and help documents for timelapse/multiview files, and here for tiled files. tif converter for Lightsheet files is implemented as a Fiji/ImageJ macro. XY-scan of 1024 × 1024 pixel images was performed with resolution 8-bit in two channels. The images were collected with EC Plan-Neofluar 100×/1.3 oil M27 and with additional digital zoom factor 2.86. Fiji/ImageJįor a free copy of Fiji (ImageJ) image processing software, click here: Fiji (Windows or Mac)Ĭlick here to be redirected to the extensive Fiji online documentationĬlick here for a link to the ImageJ 1 documentation Lightsheet. Carl Zeiss LSM 750 with LSM Software ZEN 2009 was used for 3D-imaging. The HCBI recommends frequent downloads of ZEN to ensure your software is up-to-date and compatible with files from our newest microscopesĬlick here to download the ZEN Blue Image analysis guide. Use this link to familarize yourself with any microsocpe at HCBI and setting up its acquisition parameters.įor a free copy of ZEN lite image processing software, click here: ZEN lite (Windows only).